详情

Refining Environmental DNA protocols developed for the endangered James Spinymussel (Pleurobema collina)

项目名称：	Refining Environmental DNA protocols developed for the endangered James Spinymussel (Pleurobema collina)
摘要：	The James Spinymussel (JSM) is an endangered freshwater mussel endemic to aquatic habitats in non-tidal streams of Virginia�s James River basin. Federal law requires VDOT to conduct biological surveys of the JSM before initiating projects. These conventional surveys are relatively costly and require a high degree of technical expertise. Environmental DNA (eDNA) is a genetic technique that may be used to locate, monitor, and survey the status of JSMs. Previous work developed protocols that were at least as accurate as physical sampling for the detection of JSMs within individual stream reaches. This project will focus on extending the findings of the previous study by (1) developing the lower boundaries of detection for template DNA concentration, (2) developing protocols to overcome latent inhibition of molecular genetic reactions, and (3) increasing the precision of using down-stream sampling analyses to determine population information.
状态：	Active
资金：	121898
资助组织：	Virginia Transportation Research Council
项目负责人：	Donaldson, Bridget
执行机构：	Virginia Commonwealth University
主要研究人员：	Dyer, Rodney
开始时间：	20180816
预计完成日期：	20200831
实际结束时间：	0