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Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources
| 题名： | Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources |
| 作者： | Michael P. Wolcott;Frank J. Loge;William A. Smith;Erik R. Coats;David N. Thompson; |
| 关键词： | activated sludge; denaturing gradient gel electrophoresis; DGGE; polyhydroxyalkanoates; wastewater59 - BASIC BIOLOGICAL SCIENCES; BIOREACTORS; BIOTECHNOLOGY; CARBON SOURCES; COMMUNITIES; DNA; ELECTROPHORESIS; FUNCTIONALS; POLYMERS; PRODUCTION; SLUDGES; STABILITY; THERMOPLASTICS; WASTES |
| 摘要： | Polyhydroxyalkanoates (PHAs) represent an environmentally-effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85%, 53%, and 10%of the cell dry weight from methanol-enriched pulp-and-paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Employing denaturing gradient gel electrophoresis of 16S-rDNA from PCR-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment. Most importantly, functional stability was maintained despite differing and contrasting microbial populations. |
| 报告类型： | 科技报告 |
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