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原文传递 Identification of Sildenafil (Viagra (Trade Name)) and Its Metabolite (UK-103,320) in Six Aviation Fatalities.

题名：	Identification of Sildenafil (Viagra (Trade Name)) and Its Metabolite (UK-103,320) in Six Aviation Fatalities.
作者：	JOHNSON, R. D.; LEWIS, R. J.
关键词：	AIRCRAFT ACCIDENTS; DRUGS; METABOLITES; TOXICOLOGY
摘要：	During the investigation of aviation accidents, postmortem samples from victims are submitted to the Federal Aviation Administration's Civil Aerospace Medical Institute for toxicological analysis. This report presents a rapid and reliable method for the identification and quantitation of sildenafil (Viagra(Trade Name)) and its active metabolite, UK-103,320. This procedure utilizes sildenafil-d(sub 8) as an internal standard for more accurate and reliable quantitation. The method incorporates solid-phase extraction and LC/MS/MS and MS/MS/MS utilizing an atmospheric pressure chemical ionization ion trap mass spectrometer in the positive chemical ionization mode. Solid-phase extraction provided an efficient sample extraction yielding recoveries ranging from 79 - 88%. The limit of detection for sildenafil and UK-103,320 was 0.39 and 0.19 ng/mL, respectively. The linear dynamic range for both compounds was 0.78 - 800 ng/mL. The method was employed for the determination of sildenafil and UK-103,320 in postmortem fluid and tissue specimens collected from 6 fatal aviation accident victims. The current method proved to be simple, accurate, and robust for the identification and quantitation of sildenafil and UK-103,320 in postmortem fluids and tissues.
报告类型：	科技报告