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Diversifying Midwestern Biofuel Feedstocks: Evaluation of Nitrogen Fixing Alnus & Salix Germplasm

项目名称：	Diversifying Midwestern Biofuel Feedstocks: Evaluation of Nitrogen Fixing Alnus & Salix Germplasm
摘要：	This research project will evaluate and develop woody feedstocks such as hybrid polar and alder to augment cellulosic feedstock production. Sites have been secured and plantings and seedlings purchased. Mature wood samples collected to compare to seedlings for wood structure and carbohydrate yield. Samples analyzed for ash, lignin, carbohydrate & extractive content. Currently finalizing cellulase enzymes for enzymatic hydrolysis and a standard hydrolysis process for all samples. Task 1: Characterize juvenile wood samples of Alnus. Task 2: Quantify the ash content of the juvenile Alnus samples. Task 3: Assay enzymes used for hydrolyzing biomass to determine their viability/activity level . Task 4: Compile the viability and height data from the planting sites. 3rd Quarter Report: efforts have focused on the enzymatic hydrolysis of all species of Alnus. Procedures outlined by the National Renewable Energy Laboratory for the enzymatic hydrolysis of lignocellulosic biomass have been completed, and the resulting hydrolysate is set to be analyzed by high performance liquid chromatography. This data will provide a baseline efficiency for the enzymatic hydrolysis of Alnus polysaccharides to their monomeric sugar constituents. With the baseline established, enzymatic hydrolysis efficiency gains achieved through the incorporation of a biomass pretreatment step can be properly assessed through comparative analysis.
状态：	Completed
资金：	480744.00
资助组织：	Research and Innovative Technology Administration
项目负责人：	Johnson, Shawn
执行机构：	University of Minnesota, St Paul
开始时间：	20070701
实际结束时间：	20120630
主题领域：	Energy；Transportation (General)