Trace Metals in Airborne Particulate Matter and Genomic Characterization of Associated Microorganisms: Insights into Health Effects from an Industrialized, Near-Roadway Site in Houston
| 项目名称： | Trace Metals in Airborne Particulate Matter and Genomic Characterization of Associated Microorganisms: Insights into Health Effects from an Industrialized, Near-Roadway Site in Houston |
| 摘要： | This project focuses on PM₁₀ prevalent at an air monitoring site adjacent to the Houston Ship Channel and Interstate Highway 610; Clinton Drive. The main goal of this study is to simultaneously measure major/trace metals and microorganism diversity in airborne coarse particulate matter. We will quantify vehicular contributions to PM₁₀ by analyzing aerosols’ elemental composition. Additionally, state-of-the-art next generation sequencing tools and 16S rRNA gene sequencing will be implemented to evaluate airborne microorganism diversity and prevalence in PM₁₀ in the proximity of roadways. Nine PM₁₀ samples were collected from August 10 – August 18, 2018 for analysis. Elemental analysis will be performed using high temperature microwave assisted acid digestion followed by inductively coupled plasma – mass spectrometry (ICP-MS). Next, source apportionment via the chemical mass balance model will be performed to determine the extent to which tailpipe and non-tailpipe emissions including resuspended road dust contribute to ambient PM concentrations. These experiments and modeling will be conducted by Mr. Sourav Das, a doctoral student in the Department of Civil & Environmental Engineering. Separately, Dr. Jothikumar at the Centers for Disease Control and Prevention will implement novel whole genome sequencing to evaluate microorganism diversity. Note that PM₁₀ is targeted to capture fungi. Finally, undergraduate students will review health effects literature relevant to our measurements stressing knowledge gaps. At the successful completion of this project, a novel, high-impact, high quality, “hard science” dataset on exposure of low-income underrepresented minority families to vehicular trace metals and biological aerosols proximal to a busy roadway will be generated. |
| 状态： | Active |
| 资金： | 105249 |
| 资助组织： | Office of the Assistant Secretary for Research and Technology |
| 执行机构： | Texas A&M Transportation Institute, College Station |
| 开始时间： | 20200901 |
| 预计完成日期： | 20210831 |
| 主题领域： | Environment；Highways；Society |
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