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原文传递一种稳定、抗干扰能力强的直接胆红素（氧化酶法）检测试剂及检测方法

专利名称：	一种稳定、抗干扰能力强的直接胆红素（氧化酶法）检测试剂及检测方法
摘要：	本发明涉及血清直接胆红素检测技术领域，特别涉及一种血清直接胆红素(氧化酶法)检测试剂，试剂R1中含有缓冲液，氟化钠，NAC(N‑乙酰半胱氨酸)，4‑甲苯磺酸钠，脂肪酶，抗坏血酸氧化酶，α‑环糊精，蔗糖，海藻糖，PEG‑6000，脂肪醇聚氧乙烯醚(AEO‑9)，防腐剂；试剂R2中含有缓冲液，胆红素氧化酶，蔗糖，海藻糖，PEG‑6000，脂肪醇聚氧乙烯醚(AEO‑9)，防腐剂。本试剂明显提高了测试直接胆红素时的特异性和准确性，可防止反应体系浑浊、促进反应进行，使试剂的稳定性、抗干扰能力进一步提高。
专利类型：	发明专利
国家地区组织代码：	山东;37
申请人：	济南宇鑫生物科技有限公司
发明人：	李志明;胡晓飞;李道伟;李贵雨
专利状态：	有效
申请日期：	2017-12-30T00:00:00+0800
发布日期：	2019-07-09T00:00:00+0800
申请号：	CN201711490605.3
公开号：	CN109991177A
分类号：	G01N21/31(2006.01);G;G01;G01N;G01N21
申请人地址：	250101 山东省济南市高新区工业南路51号小鸭工业园办公楼1层创新孵化中心1-115室
主权项：	1.一种血清直接胆红素（氧化酶法）检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1中含有缓冲液························································50mmol/L，氟化钠························································2 mmol/L， NAC（N-乙酰半胱氨酸）···········································0.2 mmol/L， 4-甲苯磺酸钠··················································40 mmol/L，脂肪酶························································15KU/L，抗坏血酸氧化酶················································8 KU/L， α-环糊精······················································1.5g/L，蔗糖··························································40g/L，海藻糖························································10g/L， PEG-6000······················································2g/L，脂肪醇聚氧乙烯醚（AEO-9）·······································1g/L，防腐剂························································0.2mL/L； 2）试剂R2的组分为：缓冲液························································50mmol/L，胆红素氧化酶··················································12KU/L，蔗糖··························································40g/L，海藻糖························································10g/L， PEG-6000······················································2g/L，脂肪醇聚氧乙烯醚（AEO-9）·······································1g/L，防腐剂························································0.2mL/L。 2.根据权利要求1所述的血清直接胆红素检测试剂，其特征在于试剂R1中缓冲液为25℃，pH为4.5的MES缓冲液。 3.根据权利要求1所述的血清直接胆红素检测试剂，其特征在于试剂R2中缓冲液为25℃，pH为4.5的MES缓冲液。 4.根据权利要求1所述的血清直接胆红素检测试剂，其特征在于试剂R1和R2中表面活性剂为脂肪醇聚氧乙烯醚（AEO-9）。 5.根据权利要求1所述的血清直接胆红素检测试剂，其特征在于所述防腐剂为Proclin300。 6.一种使用权利要求1-5中任一项所述的血清直接胆红素检测试剂来检测血清直接胆红素的检测方法，其特征在于使用全自动生化分析仪利用终点法进行测定，检测主波长为450nm。 7.根据权利要求6所述的检测方法，其特征在于R1试剂和R2试剂的比例为3:1。
所属类别：	发明专利